脂质对实验性自身免疫性神经炎抗原决定簇构象的影响。

Effect of lipids on the conformation of an antigenic determinant for experimental autoimmune neuritis.

作者信息

Shin H C

机构信息

Protein Engineering Laboratory, Hanhyo Institute of Technology, Taejon, Korea.

出版信息

Biochem Mol Biol Int. 1998 Apr;44(4):771-6. doi: 10.1080/15216549800201812.

DOI:10.1080/15216549800201812

PMID:9584990

Abstract

The conformation of SP-26, the synthetic peptide (residues 53-78) of myelin P2 protein that causes experimental autoimmune neuritis (EAN) in the peripheral nervous system, has been studied in lipid environments and in water/trifluoroethanol (TFE) mixture using circular dichroism (CD) spectroscopy and Fourier transform infra-red (FTIR) spectroscopy. SP-26 becomes more structured on binding with micelles formed from anionic lipids and in the presence of TFE. On the other hand, zwitterionic lipids have a denaturing effect or no effect on the conformation of SP-26. The present results suggest that the disease-modulating effects of various lipids are closely related to their ability to induce conformational change in the antigenic region of P2 protein.

摘要

髓磷脂P2蛋白的合成肽（第53 - 78位氨基酸残基）SP - 26可引发外周神经系统的实验性自身免疫性神经炎（EAN），已利用圆二色光谱（CD）和傅里叶变换红外光谱（FTIR）在脂质环境以及水/三氟乙醇（TFE）混合物中对其构象进行了研究。SP - 26在与由阴离子脂质形成的胶束结合时以及在TFE存在的情况下，结构变得更加规整。另一方面，两性离子脂质对SP - 26的构象具有变性作用或无影响。目前的结果表明，各种脂质的疾病调节作用与其诱导P2蛋白抗原区域构象变化的能力密切相关。