Song X, Norman A W
Department of Biochemistry, University of California-Riverside, 92521, USA.
Leuk Res. 1998 Jan;22(1):69-76. doi: 10.1016/s0145-2126(97)00054-4.
Both 1alpha,25-dihydroxyvitamin D3 [1alpha,25(OH)2D3] and phorbol 12-myristate 13-acetate [PMA] are cytodifferentiation agents regulating myeloid cell line differentiation along the monocyte/macrophage pathway. Alkaline phosphatase (ALP) activity has been reported to be absent or present at low levels in monocytes/macrophages. In the present study, the acute promyelocytic leukemia cell line (NB4) was found to undergo monocyte/macrophage differentiation and strongly express ALP activity after exposure to the combination of 1alpha,25(OH)2D3 and PMA treatment. The ALP stimulation was both dose- and time-dependent with the ED50 doses for 1alpha,25(OH)2D3 and PMA at 2 x 10(-10) M and 4 x 10(-11) M, respectively. Assessment of the cell morphology via cell adherence, phagocytosis and ALP staining confirmed that NB4 cells treated by 1alpha,25(OH)2D3 plus PMA showed macrophage characteristics, but were also strongly stained with ALP. The present study is the first report that NB4 cells express ALP activity as a consequence of combination treatment with 1alpha,25(OH)2D3 and PMA.
1α,25 - 二羟基维生素D3[1α,25(OH)2D3]和佛波酯12 - 肉豆蔻酸酯13 - 乙酸酯[PMA]都是调节髓系细胞系沿单核细胞/巨噬细胞途径分化的细胞分化剂。据报道，单核细胞/巨噬细胞中不存在碱性磷酸酶(ALP)活性或其活性水平较低。在本研究中，发现急性早幼粒细胞白血病细胞系(NB4)在暴露于1α,25(OH)2D3和PMA联合处理后会发生单核细胞/巨噬细胞分化并强烈表达ALP活性。ALP刺激具有剂量和时间依赖性，1α,25(OH)2D3和PMA的ED50剂量分别为2×10(-10)M和4×10(-11)M。通过细胞黏附、吞噬作用和ALP染色对细胞形态进行评估，证实经1α,25(OH)2D3加PMA处理的NB4细胞具有巨噬细胞特征，并且也被ALP强烈染色。本研究是首次报道NB4细胞因1α,25(OH)2D3和PMA联合处理而表达ALP活性。
