通过化学和酶促反应联合使用立体选择性地合成13C/2H双标记的DNA寡聚物。
Synthesis of stereoselectively 13C/2H-doubly labeled DNA oligomers by the combined use of chemical and enzymatic reactions.
作者信息
Ono A, Oogo Y, Tate S, Ono A, Kainosho M
机构信息
Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, Japan.
出版信息
Nucleic Acids Symp Ser. 1997(37):73-4.
PMID:9586005
Abstract
(2'S,5'S)-(1',2',3',4',5'-13C5;2',5'-2H2-thymidine, prepared via labeled ribosyl thymidine as described in the accompanying paper, has been successfully converted into isotopically labeled dA, dG, and dC using enzymatic transdeoxyribosylation reactions.
摘要
（2'S，5'S）-（1'，2'，3'，4'，5'-13C5；2'，5'-2H2）-胸苷，通过随附论文中所述的标记核糖基胸苷制备，已使用酶促转脱氧核糖基化反应成功转化为同位素标记的dA、dG和dC。
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