Ono A, Takaishi N, Tatebe M, Ono A, Tate S, Kainosho M
Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, Japan.
Nucleic Acids Symp Ser. 1997(37):75-6.
A systematic synthesis of pyrimidine nucleosides with 13C-labels only at specific atoms in both the base and sugar moieties has been developed in order to obtain conformational information about the glycosidic bond through measurement of the accurate vicinal coupling constants between H1' and C2/C6. For this purpose, 13C labels at three positions, namely C1', C2, and C6, are essential. We have synthesized selectively multiply labeled nucleosides, such as [2, 1'-13C2]-thymidine, [2, 1'-13C2]-2'-deoxycytidine, [6, 1'-13C2]-thymidine, and [6, 1'-13C2]-2'-deoxycytidine. These nucleosides will be useful to determine the relative orientation of the base and sugar moieties.
已开发出一种仅在嘧啶核苷的碱基和糖部分的特定原子上带有¹³C标记的系统合成方法，以便通过测量H1'与C2/C6之间准确的邻位偶合常数来获取有关糖苷键的构象信息。为此，在三个位置（即C1'、C2和C6）的¹³C标记至关重要。我们已经选择性地合成了多重标记的核苷，例如[2, 1'-¹³C₂]-胸苷、[2, 1'-¹³C₂]-2'-脱氧胞苷、[6, 1'-¹³C₂]-胸苷和[6, 1'-¹³C₂]-2'-脱氧胞苷。这些核苷将有助于确定碱基和糖部分的相对取向。
