通过双链DNA中的空穴跳跃实现的-GG-特异性切割。
-GG- specific cleavage via hole hopping through double-stranded DNA.
作者信息
Nakamura T, Dohno C, Nakatani K, Saito I
机构信息
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Japan.
出版信息
Nucleic Acids Symp Ser. 1997(37):87-8.
We examined the photoinduced DNA cleavage reaction of 32P-end labeled oligodeoxyribonucleotides which contain 5'-TGGGC-3', 5'-CGGGC-3', and 5'-TGGGT-3'. As for 5'-TGGGC-3' and 5'-TGGGT-3', the cleavage occurred most strongly at the middle of 5'-GGG-3' step, whereas the strongest cleavage occurred at the 5' side of 5'-GGG-3' in 5'-CGGGC-3' step. These results showed the 5' side base of 5'-GGG-3' step was very important for the sequence selectivity of 5'-GGG-3' cleavage.
我们研究了含5'-TGGGC-3'、5'-CGGGC-3'和5'-TGGGT-3'的32P末端标记的寡脱氧核糖核苷酸的光诱导DNA切割反应。对于5'-TGGGC-3'和5'-TGGGT-3'，切割在5'-GGG-3'步的中间位置最为强烈，而在5'-CGGGC-3'步中，最强的切割发生在5'-GGG-3'的5'侧。这些结果表明，5'-GGG-3'步的5'侧碱基对于5'-GGG-3'切割的序列选择性非常重要。
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