多卡霉素A-偏端霉素A异二聚体的协同烷基化作用。
Cooperative alkylation by duocarmycin A-distamycin A heterodimer.
作者信息
Ozeki Y, Sugiyama H, Saito I
机构信息
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Japan.
出版信息
Nucleic Acids Symp Ser. 1997(37):91-2.
Duo A (Duo) normally alkylates adenine N3 at the 3' end of A + T-rich sequence in DNA. The addition of another minor groove binder, dist A (Dist), dramatically modulate the site of DNA alkylation by Duo with great acceleration of the reaction rate. In order to examine the mode of alkylation, the kinetics of the reaction under various conditions were examined. Based on the simulation of experimental data, a new reaction pathway was proposed.
双功能试剂A（Duo）通常会使DNA中富含A+T序列3'端的腺嘌呤N3发生烷基化。添加另一种小沟结合剂dist A（Dist），能显著改变Duo对DNA的烷基化位点，并极大地加快反应速率。为了研究烷基化模式，我们考察了各种条件下反应的动力学。基于实验数据的模拟，提出了一条新的反应途径。
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