Suzuki T, Nakamura T, Kanaori K, Tajima K, Makino K
Institute of Advanced Energy, Kyoto University, Uji, Japan.
Nucleic Acids Symp Ser. 1997(37):241-2.
Using reversed phase (RP) HPLC analysis, we reinvestigated the reaction of dCyd with nitrous acid. When 10 mM 2'-deoxycytidine (dCyd) was incubated with 100 mM NaNO2 in 1.0 M acetate buffer (pH 3.7) at 37 degrees C, an unknown product (referred to compound 1) was detected. The time course of the yield of compound 1 showed profile characteristic of an intermediate. Under the physiological conditions, compound 1 was converted exclusively to dUrd with a life time of ca. 300 h. Elucidation of genotoxic effects of compound 1 may be an important subject of the future study.
