Nishinaka T, Ito Y, Yokoyama S, Shibata T
Cellular and Molecular Biology Laboratory, Saitama, Japan.
Nucleic Acids Symp Ser. 1997(37):269-70.
We recently reported an NMR study indicating that the DNA structure bound to RecA protein contains a novel deoxyribose-base stacking interaction. Although the final structure calculated by the simulated annealing was well defined, the type of sugar puckers was neither pure C2'-endo nor C3'-endo, but something in between. This implies that the sugar moieties in the RecA-bound state actually take an intermediate puckering, but we can not exclude the possibility that RecA-bound DNA takes multiple conformations between the S-type and N-type of sugar puckers. In either case, the DNA structure within RecA filaments shows a unique property that is distinct from canonical DNA structures.
我们最近报道了一项核磁共振研究，该研究表明与RecA蛋白结合的DNA结构包含一种新型的脱氧核糖-碱基堆积相互作用。尽管通过模拟退火计算得到的最终结构定义明确，但糖环构象的类型既不是纯的C2'-内型也不是C3'-内型，而是介于两者之间。这意味着处于与RecA结合状态的糖部分实际上采取了一种中间构象，但我们不能排除与RecA结合的DNA在S型和N型糖环构象之间采取多种构象的可能性。在任何一种情况下，RecA细丝内的DNA结构都显示出一种与经典DNA结构不同的独特性质。
