Uzcátegui N, Flores J, Liprandi F, Pujol F H
Laboratorio de Biología de Virus, Universidad Central de Venezuela, Caracas, Venezuela.
Invest Clin. 1998 Mar;39(1):19-28.
Detection of IgM anti-core (anti-HBcAg) antibodies of Hepatitis B Virus (HBV) is an useful marker for hepatitis B virus (HBV) acute infection. The aim of this study was to perform an immunodiagnostic assay for the detection of IgM anti-HBcAg antibodies. Hepatitis B core antigen (HBcAg) was produced by a recombinant clone of Escherichia coli and used for the development of the immunoassay. An IgM capture enzyme immunoassay (EIA) was selected for the detection of IgM anti-HBcAg antibodies. A total of 110 human plasma or sera were tested by the capture EIA and a commercial assay. The capture EIA yielded 99% of sensitivity and 93% specificity, when compared with the commercial test. The capture EIA developed here is of interest for epidemiological studies, particularly for endemic regions in South America.
检测乙型肝炎病毒（HBV）的IgM抗核心（抗-HBcAg）抗体是乙型肝炎病毒（HBV）急性感染的一项有用标志物。本研究的目的是进行一项免疫诊断检测以检测IgM抗-HBcAg抗体。乙型肝炎核心抗原（HBcAg）由大肠杆菌的重组克隆产生，并用于免疫检测的开发。选择IgM捕获酶免疫测定法（EIA）来检测IgM抗-HBcAg抗体。通过捕获EIA和一种商业检测法对总共110份人血浆或血清进行了检测。与商业检测法相比，捕获EIA的灵敏度为99%，特异性为93%。这里开发的捕获EIA对于流行病学研究，特别是南美洲的流行地区具有重要意义。
