Rodgers J B, Vasconez H C, Wells M D, DeLuca P P, Faugere M C, Fink B F, Hamilton D
Department of Surgery, University of Kentucky, Lexington 40536-0284, USA.
J Craniofac Surg. 1998 Mar;9(2):147-53. doi: 10.1097/00001665-199803000-00012.
We have developed a lyophilized bone morphogenetic protein (BMP) delivery device that can be formulated to control release over 2 to 8 weeks. Bioerodible poly (d,l lactide-co-glycolide) particles loaded with 90 micrograms recombinant human BMP-2 were suspended in either carboxymethylcellulose (CMC) or methylcellulose (MC) implants. Plain CMC and MC implants served as controls, as did a nonimplanted group. A total of 40 rabbits was evaluated histologically 2, 4, or 8 weeks after receiving circular full-thickness 15-mm calvarial defects. MC appeared to prevent prolapse of periosteum and dura into the defects and did not elicit bone growth. Addition of BMP improved the result. CMC implants appeared to encourage bone growth even in the absence of BMP. When BMP was added, new bone formed earlier. CMC may influence new bone formation because it is hydrophilic. MC is less hydrophilic and may cause undue inflammation. Either can be combined with BMP to produce unitary devices that are easy to make and use.
我们已经开发出一种冻干骨形态发生蛋白（BMP）递送装置，该装置可以被制备成能在2至8周内实现控释。载有90微克重组人BMP-2的可生物降解聚（d，l丙交酯-共-乙交酯）颗粒悬浮于羧甲基纤维素（CMC）或甲基纤维素（MC）植入物中。普通CMC和MC植入物作为对照，未植入组也作为对照。总共40只兔子在接受直径15毫米的圆形全层颅骨缺损后2、4或8周进行组织学评估。MC似乎能防止骨膜和硬脑膜脱垂至缺损处，且不会引发骨生长。添加BMP改善了结果。即使在没有BMP的情况下，CMC植入物似乎也能促进骨生长。当添加BMP时，新骨形成得更早。CMC可能因其亲水性而影响新骨形成。MC亲水性较差，可能会引起过度炎症。两者都可以与BMP结合，制成易于制备和使用的单一装置。
