Lvov Y, Onda M, Ariga K, Kunitake T
Supermolecules Project, JST, Kurume Research Center, Fukuoka, Japan.
J Biomater Sci Polym Ed. 1998;9(4):345-55. doi: 10.1080/09205063.1998.9753060.
As a means of preparation of biocompatible molecular surfaces, an alternate assembly of charged polysaccharides and oppositely-charged synthetic polymers was conducted. Cationic chitosan was assembled alternately with anionic poly(sodium styrenesulfonate) (PSS) at pH 4. Regular film growth and its dependence on ionic strength were detected by the quartz crystal microbalance (QCM) method. Averaged film thicknesses for the chitosan + PSS layer were 15, 31, 46, and 69 A, respectively, when 0, 0.25, 0.5, and 1 M of NaCl was contained in aqueous chitosan. Adsorption of chitosan did not reach saturation in 20 min at 0 M NaCl, while the adsorption became saturated within 6 min with 0.25 M NaCl. Anionic sodium chondroitin sulfate was also assembled in alternation with cationic poly(dimethyldiallylammonium chloride) (PDDA) at pH 6.5. The adsorption of chondroitin sulfate was less sensitive to ionic strength. Surface morphology of chitosan-PSS films was investigated by non-contact atomic force microscopy (AFM) observation. Maximum height difference and Ra value for a 1000 x 1000 nm area were 11 and 0.69 nm, respectively, indicating the formation of a molecularly flat surface by alternate layer-by-layer adsorption.
作为一种制备生物相容性分子表面的方法，进行了带电荷多糖和带相反电荷的合成聚合物的交替组装。在pH值为4的条件下，将阳离子壳聚糖与阴离子聚苯乙烯磺酸钠（PSS）交替组装。通过石英晶体微天平（QCM）方法检测了规则的膜生长及其对离子强度的依赖性。当壳聚糖水溶液中分别含有0、0.25、0.5和1 M的NaCl时，壳聚糖+PSS层的平均膜厚度分别为15、31、46和69 Å。在0 M NaCl时，壳聚糖在20分钟内吸附未达到饱和，而在0.25 M NaCl时，吸附在6分钟内达到饱和。在pH值为6.5的条件下，阴离子硫酸软骨素也与阳离子聚二甲基二烯丙基氯化铵（PDDA）交替组装。硫酸软骨素的吸附对离子强度不太敏感。通过非接触原子力显微镜（AFM）观察研究了壳聚糖-PSS膜的表面形态。对于1000×1000 nm的区域，最大高度差和Ra值分别为11和0.69 nm，表明通过交替逐层吸附形成了分子平坦表面。
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