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通过水解以及壳聚糖和硫酸软骨素的层层组装对聚对苯二甲酸乙二酯进行表面改性,以构建对人内皮细胞具有细胞相容性的层。

Surface modification of poly(ethylene terephthalate) via hydrolysis and layer-by-layer assembly of chitosan and chondroitin sulfate to construct cytocompatible layer for human endothelial cells.

作者信息

Liu Yunxiao, He Tao, Gao Changyou

机构信息

Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.

出版信息

Colloids Surf B Biointerfaces. 2005 Dec 10;46(2):117-26. doi: 10.1016/j.colsurfb.2005.09.005. Epub 2005 Nov 9.

Abstract

Surface modification of poly(ethylene terephthalate) (PET) film was performed by surface hydrolysis and layer-by-layer (LBL) assembly followed a mechanism of electrostatic adsorption of oppositely charged polymers, exemplified with chitosan and chondroitin sulfate (CS). Hydrolysis of PET in concentrated alkaline solution produced a carboxyl-enriched surface. The changes of weight loss and surface chemistry, morphology and wettability were monitored and verified by UV-vis spectroscopy, atomic force microscopy (AFM) and water contact angle. Assembly of positively charged chitosan and negatively charged CS was then conducted in a LBL manner to create multilayers on the hydrolyzed PET film. The process of layer growth and oscillation of surface wettability were monitored by UV-vis spectroscopy and water contact angle measurement, respectively. In vitro cell culture revealed that the adherence of endothelial cells was significantly enhanced on the biomacromolecules-modified PET film with preserved endothelial cell function, in particular on those assembled with larger number of chitosan/CS layers. However, with regard to cell proliferation and viability properties after cultured for 4 days, minor difference was determined between the modified and the unmodified PET films.

摘要

通过表面水解和层层(LBL)组装对聚对苯二甲酸乙二酯(PET)薄膜进行表面改性,其遵循带相反电荷聚合物的静电吸附机制,以壳聚糖和硫酸软骨素(CS)为例。在浓碱性溶液中对PET进行水解可产生富含羧基的表面。通过紫外可见光谱、原子力显微镜(AFM)和水接触角监测并验证了重量损失、表面化学、形态和润湿性的变化。然后以LBL方式进行带正电荷的壳聚糖和带负电荷的CS的组装,以在水解的PET薄膜上形成多层结构。分别通过紫外可见光谱和水接触角测量监测层生长过程和表面润湿性的振荡。体外细胞培养表明,在具有保留的内皮细胞功能的生物大分子改性PET薄膜上,内皮细胞的粘附显著增强,特别是在组装有更多壳聚糖/CS层的薄膜上。然而,对于培养4天后的细胞增殖和活力特性,改性PET薄膜和未改性PET薄膜之间存在微小差异。

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