东莨菪碱相关抗胆碱能药物的胶束电动色谱法

Micellar electrokinetic chromatography of scopolamine-related anticholinergics.

作者信息

Wu H L, Huang C H, Chen S H, Wu S M

机构信息

Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical College, Taiwan.

出版信息

J Chromatogr A. 1998 Apr 3;802(1):107-13. doi: 10.1016/s0021-9673(97)01141-2.

DOI:10.1016/s0021-9673(97)01141-2

PMID:9588013

Abstract

A simple micellar electrokinetic chromatography (MEKC) method is described for the separation of scopolamine N-oxide hydrobromide (SO), scopolamine hydrobromide (SH), scopolamine N-methylbromide (SM) and scopolamine N-butylbromide (SB), and for the quantitation of SH, SM and SB (using SO as an internal standard). The analysis of these drugs was performed in a phosphate buffer (30 mM; pH 7.00) with sodium dodecyl sulfate (SDS) (30 mM) as an anionic surfactant. Several parameters affecting the separation of the drugs were studied, including the concentrations of the buffer and SDS. The stability of the drugs in the phosphate buffer (pH 7.00) was also examined. Partial application of the method to the determination of scopolamine N-butylbromide in tablets proved to be feasible.

摘要

描述了一种简单的胶束电动色谱（MEKC）方法，用于分离氢溴酸东莨菪碱氮氧化物（SO）、氢溴酸东莨菪碱（SH）、东莨菪碱甲基溴（SM）和东莨菪碱丁基溴（SB），并用于定量测定SH、SM和SB（以SO作为内标）。这些药物的分析在含有十二烷基硫酸钠（SDS）（30 mM）作为阴离子表面活性剂的磷酸盐缓冲液（30 mM；pH 7.00）中进行。研究了影响药物分离的几个参数，包括缓冲液和SDS的浓度。还考察了药物在磷酸盐缓冲液（pH 7.00）中的稳定性。该方法部分应用于片剂中东莨菪碱丁基溴的测定，结果证明是可行的。