File S E, Gonzalez L E, Andrews N
United Medical and Dental Schools of Guy's and St. Thomas' Hospitals, Division of Pharmacology, London, United Kingdom.
Behav Neurosci. 1998 Apr;112(2):352-9. doi: 10.1037//0735-7044.112.2.352.
Dorsal hippocampal cholinergic modulation of behavior in different tests of anxiety was investigated by direct injection of the muscarinic M1 and M2 receptor antagonists, pirenzepine and gallamine, and the nicotinic receptor antagonist mecamylamine. In the social interaction test, the anxiogenic effect of pirenzepine (30-100 ng) provided evidence for a tonic cholinergic anxiolytic action mediated by postsynaptic M1 receptors. The anxiogenic action of mecamylamine (30 and 100 ng) was most likely mediated by its action of presynaptic nicotinic receptors to reduce acetylcholine release. Gallamine (10-1,000 ng) was without effect, suggesting that M2 receptors in this brain region do not play a significant role in this behavioral test. On Trial 1 in the elevated plus-maze, the receptor antagonists were without any effect, but in those with a previous 5-min experience of the plus-maze pirenzepine and mecamylamine had anxiogenic effects in the dose range of 30-300 ng; gallamine (100 and 300 ng) was without significant effect.
通过直接注射毒蕈碱M1和M2受体拮抗剂哌仑西平和加拉明以及烟碱受体拮抗剂美加明，研究了背侧海马胆碱能对不同焦虑测试中行为的调节作用。在社交互动测试中，哌仑西平（30 - 100纳克）的致焦虑作用为突触后M1受体介导的紧张性胆碱能抗焦虑作用提供了证据。美加明（30和100纳克）的致焦虑作用很可能是通过其对突触前烟碱受体的作用来减少乙酰胆碱释放介导的。加拉明（10 - 1000纳克）没有效果，表明该脑区的M2受体在该行为测试中不发挥重要作用。在高架十字迷宫试验的第1次试验中，受体拮抗剂没有任何作用，但在那些之前有5分钟高架十字迷宫体验的试验中，哌仑西平和美加明在30 - 300纳克的剂量范围内有致焦虑作用；加拉明（100和300纳克）没有显著作用。
