Bentz B G, Haines G K, Hanson D G, Radosevich J A
Department of Otolaryngology-Head and Neck Surgery and the Robert H. Lurie Cancer Center, Northwestern University Medical Center, Chicago, Illinois, USA.
Head Neck. 1998 Jul;20(4):304-9. doi: 10.1002/(sici)1097-0347(199807)20:4<304::aid-hed4>3.0.co;2-2.
Nitric oxide (NO.) has been implicated in the process of carcinogenesis in various organs. This study was designed to investigate the expression of endothelial constitutive nitric oxide synthase (ecNOS) in normal and neoplastic salivary tissues.
Paraffin-embedded tissue from 48 salivary tumors and adjacent non-neoplastic tissue was immunohistochemically evaluated for both frequency (percentage) and intensity (1-4+) of staining using a commercially available anti-ecNOS monoclonal antibody.
Expression of ecNOS was predominantly localized to vascular endothelium, skeletal muscle, and to salivary duct luminal epithelium in normal salivary tissue (n = 37). All salivary tumors demonstrated at least 1 + cytoplasmic staining for ecNOS without apparent correlation to most clinical parameters. A tendency toward increased frequency and intensity of ecNOS expression in oncocytic cells, relative to cells with myoepithelial or acinar differentiation, was noted.
Expression of ecNOS is localized to the luminal cells of normal salivary ducts. Limited expression of ecNOS was found in all the salivary gland tumors examined. This suggests a common histogenesis for this diverse group of tumors, which may reflect different degrees of differentiation toward luminal duct epithelium. The possible role of ecNOS and NO. in salivary gland carcinogenesis is intriguing and warrants further study.
一氧化氮（NO.）已被认为参与了多种器官的致癌过程。本研究旨在调查内皮型一氧化氮合酶（ecNOS）在正常和肿瘤性唾液组织中的表达情况。
使用市售的抗ecNOS单克隆抗体，对48例唾液腺肿瘤及相邻非肿瘤组织的石蜡包埋组织进行免疫组织化学评估，检测染色频率（百分比）和强度（1 - 4+）。
ecNOS的表达主要定位于正常唾液组织（n = 37）的血管内皮、骨骼肌和唾液导管腔上皮。所有唾液腺肿瘤均显示ecNOS至少有1 +的细胞质染色，且与大多数临床参数无明显相关性。相对于具有肌上皮或腺泡分化的细胞，观察到嗜酸性细胞中ecNOS表达的频率和强度有增加的趋势。
ecNOS的表达定位于正常唾液导管的腔面细胞。在所检查的所有唾液腺肿瘤中均发现ecNOS表达有限。这表明这一不同类型肿瘤具有共同的组织发生学，这可能反映了向导管腔上皮不同程度的分化。ecNOS和NO.在唾液腺癌发生中的可能作用很有趣，值得进一步研究。
