Hénaut A, Lisacek F, Nitschké P, Moszer I, Danchin A
Université de Versailles Saint Quentin, France.
Electrophoresis. 1998 Apr;19(4):515-27. doi: 10.1002/elps.1150190411.
Present availability of the genomic text of bacteria allows assignment of biological known functions to many genes (typically, half of the genome's gene content). It is now time to try and predict new unexpected functions, using inductive procedures that allow correlating the content of the genomic text to possible biological functions. We show here that analysis of the genomes of Escherichia coli and Bacillus subtilis for the distribution of AGCT motifs predicts that genes exist for which the mRNA molecule can be translated as several different proteins synthesized after ribosomal frameshifting or hopping. Among these genes we found that several coded for the same function in E. coli and B. subtilis. We analyzed in depth the situation of the infB gene (experimentally known to specify synthesis of several proteins differing in their translation starts), the aceF/pdhC gene, the eno gene, and the rplI gene. In addition, genes specific to E. coli were also studied: ompA, ompFand tolA (predicting epigenetic variation that could help escape infection by phages or colicins).
目前可获取的细菌基因组文本使得许多基因（通常占基因组基因含量的一半）能够被赋予已知的生物学功能。现在是时候尝试预测新的意外功能了，采用归纳程序，使基因组文本内容与可能的生物学功能相关联。我们在此表明，对大肠杆菌和枯草芽孢杆菌基因组中AGCT基序分布的分析预测，存在一些基因，其mRNA分子可通过核糖体移码或跳跃后翻译为几种不同的蛋白质。在这些基因中，我们发现有几个在大肠杆菌和枯草芽孢杆菌中编码相同的功能。我们深入分析了infB基因（实验已知可指定几种翻译起始不同的蛋白质合成）、aceF/pdhC基因、eno基因和rplI基因的情况。此外，还研究了大肠杆菌特有的基因：ompA、ompF和tolA（预测可能有助于逃避噬菌体或大肠杆菌素感染的表观遗传变异）。
