Chua P R, Roeder G S
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-8103, USA.
Cell. 1998 May 1;93(3):349-59. doi: 10.1016/s0092-8674(00)81164-2.
We describe the identification and characterization of the Saccharomyces cerevisiae ZIP2 gene, which encodes a novel meiosis-specific protein essential for synaptonemal complex formation. In the zip2 mutant, chromosomes are homologously paired but not synapsed. The Zip2 protein localizes to discrete foci on meiotic chromosomes; these foci correspond to sites of convergence between paired homologs that are believed to be sites of synapsis initiation. Localization of Zip2p requires the initiation of meiotic recombination. In a mutant defective in double-strand break repair, Zip2p colocalizes with proteins involved in double-strand break formation and processing. We propose that Zip2p promotes the initiation of chromosome synapsis and that localization of Zip2p to sites of interhomolog recombination ensures synapsis between homologous chromosomes.
我们描述了酿酒酵母ZIP2基因的鉴定和特征，该基因编码一种对联会复合体形成至关重要的新型减数分裂特异性蛋白。在zip2突变体中，染色体同源配对但未联会。Zip2蛋白定位于减数分裂染色体上的离散位点；这些位点对应于配对同源物之间的汇聚位点，据信这些位点是联会起始位点。Zip2p的定位需要减数分裂重组的起始。在双链断裂修复缺陷的突变体中，Zip2p与参与双链断裂形成和加工的蛋白质共定位。我们提出Zip2p促进染色体联会的起始，并且Zip2p定位于同源重组位点可确保同源染色体之间的联会。
