Wu Z, Zhang Y, Liu D
Department of Urology, Huashan Hospital, Shanghai Medical University.
Zhonghua Wai Ke Za Zhi. 1996 Nov;34(11):645-7.
Reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot hybridization were used to detect exon V6-containing isoforms of CD44 gene in 42 surgical bladder specimens (20 cases of bladder cancer, 10 cases of corresponding normal bladder tissue, 8 non-neoplastic bladder tissue from the benign prostatic hyperplasia, and 4 cystitis). All samples of bladder cancer tissue showed over-expression of many abnormal alternatively-spliced products containing transcripts from exon V6 of the CD44 gene except those from 22 non-neoplastic tissue. Furthermore, the band pattern permitted differentiation between the 2 cases of metastatic bladder tumors and the 18 cases of non metastatic tissue. Our results suggest that there is a remarkable relationship between the abnormal CD44 gene expression (inappropriate splicing and overexpression) and malignant phenotype and metastasis of bladder cancer. The CD44 splice variants may prove to be a more ideal tumor marker for the early diagnosis of bladder cancer and for the early detection of metastatic potential in surgical biopsy specimens, which deserves further evaluation.
采用逆转录聚合酶链反应（RT-PCR）和Southern印迹杂交技术，检测42份手术切除的膀胱标本（20例膀胱癌、10例相应的正常膀胱组织、8例来自良性前列腺增生的非肿瘤性膀胱组织和4例膀胱炎组织）中含CD44基因外显子V6的异构体。除22份非肿瘤组织外，所有膀胱癌组织样本均显示出许多异常可变剪接产物的过表达，这些产物包含来自CD44基因外显子V6的转录本。此外，条带模式有助于区分2例转移性膀胱肿瘤和18例非转移性组织。我们的结果表明，CD44基因异常表达（不适当剪接和过表达）与膀胱癌的恶性表型和转移之间存在显著关系。CD44剪接变体可能被证明是一种更理想的肿瘤标志物，用于膀胱癌的早期诊断和手术活检标本中转移潜能的早期检测，值得进一步评估。
