Hornung D, Lebovic D I, Shifren J L, Vigne J L, Taylor R N
Reproductive Endocrinology Center, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, USA.
Fertil Steril. 1998 May;69(5):909-15. doi: 10.1016/s0015-0282(98)00044-2.
To analyze directional vascular endothelial growth factor (VEGF) secretion in polarized human endometrial epithelial cell cultures. VEGF has distinct distribution patterns in human endometrium. Stromal cells are diffusely positive for VEGF messenger RNA and protein, whereas glandular epithelium shows focal VEGF immunostaining at the apical surface. The epithelial distribution suggests that VEGF is secreted into gland lumina, potentially influencing the nutrition and/or apposition of the developing blastocyst.
Controlled in vitro study of protein secretion by polarized endometrial epithelial cells established on polyethylene filters.
University hospital.
PATIENT(S): Endometrial biopsies were obtained from healthy, ovulatory women undergoing elective surgery.
INTERVENTION(S): Primary endometrial epithelial cells were cultured.
MAIN OUTCOME MEASURE(S): VEGF mRNA and protein production were measured in polarized cells. The vectorial secretion of VEGF was determined.
RESULT(S): VEGF production by endometrial epithelial cells was verified by Northern blotting and immunoassays of conditioned media. The mean (+/-SD) apical secretion of VEGF was 3.9 +/- 1.4 ng per 10(5) cells every 48 hours and the mean (+/-SD) basal secretion was 0.8 +/- 0.2 ng per 10(5) cells every 48 hours. In contrast, the apical and basal secretion of a soluble cellular isoform of fibronectin were 2.76 +/- 0.96 ng per 10(5) cells every 48 hours and 2.64 +/- 1.79 ng per 10(5) cells every 48 hours, respectively.
CONCLUSION(S): VEGF is secreted preferentially into the lumina of endometrial glands. Apical VEGF may be an endometrial signal for blastocyst development or implantation.
分析极化的人子宫内膜上皮细胞培养物中血管内皮生长因子（VEGF）的定向分泌。VEGF在人子宫内膜中有不同的分布模式。基质细胞对VEGF信使核糖核酸和蛋白质呈弥漫性阳性，而腺上皮在顶端表面显示局灶性VEGF免疫染色。上皮分布表明VEGF分泌到腺腔中，可能影响发育中的胚泡的营养和/或附着。
对在聚乙烯滤器上建立的极化子宫内膜上皮细胞的蛋白质分泌进行体外对照研究。
大学医院。
从接受择期手术的健康排卵妇女获取子宫内膜活检组织。
培养原代子宫内膜上皮细胞。
测量极化细胞中VEGF信使核糖核酸和蛋白质的产生。确定VEGF的向量分泌。
通过Northern印迹法和条件培养基免疫测定法验证了子宫内膜上皮细胞产生VEGF。VEGF的平均（±标准差）顶端分泌量为每48小时每10⁵个细胞3.9±1.4 ng，平均（±标准差）基底分泌量为每48小时每10⁵个细胞0.8±0.2 ng。相比之下，纤连蛋白可溶性细胞异构体的顶端和基底分泌量分别为每48小时每10⁵个细胞2.76±0.96 ng和每48小时每10⁵个细胞2.64±1.79 ng。
VEGF优先分泌到子宫内膜腺腔中。顶端VEGF可能是胚泡发育或着床的子宫内膜信号。
