Phenol extraction of 28 S ribosomal RNA from rat liver cytoplasm.
作者信息
Dabeva M D
出版信息
Prep Biochem. 1976;06(04):285-94. doi: 10.1080/00327487608061619.
PMID:959178
Abstract
A simple and reproducible phenol method for the isolation of 28 S ribosomal RNA from rat liver cytoplasm, free from poly(A)+/-RNA is described. The procedure is based on the observation that at lower pH of the homogenate (pH 5.5) 28 S ribosomal RNA is extracted, while 18 S ribosomal RNA remains in the interphase layer.
摘要
相似文献
1
Phenol extraction of 28 S ribosomal RNA from rat liver cytoplasm.
Prep Biochem. 1976;06(04):285-94. doi: 10.1080/00327487608061619.
2
[Minor cytoplasmic RNA: demonstration and characterization of a chicken 21 S RNA].[微小细胞质RNA：鸡21S RNA的证实与特性分析]
Biochimie. 1972;54(8):1031-40. doi: 10.1016/s0300-9084(72)80054-3.
3
A sequential extraction method for RNA from rabbit liver.一种从兔肝脏中提取RNA的连续提取方法。
Acta Chem Scand B. 1978;B32(9):671-8. doi: 10.3891/acta.chem.scand.32b-0671.
4
Agar gel chromatography of rat liver ribosomal ribonucleic acids.大鼠肝脏核糖体核糖核酸的琼脂凝胶色谱法。
Anal Biochem. 1975 Aug;67(2):462-7. doi: 10.1016/0003-2697(75)90319-x.
5
Chromatographic separation of rat liver ribonucleic acids as constituents of cytoplasmic ribonucloprotein complexes.大鼠肝脏核糖核酸作为细胞质核糖核蛋白复合物成分的色谱分离
FEBS Lett. 1972 Jun 15;23(2):254-6. doi: 10.1016/0014-5793(72)80354-5.
6
Absorption of rat liver ribosomal ribonucleic acids on agar gels.
Biochimie. 1975;57(6-7):841-2. doi: 10.1016/s0300-9084(75)80061-7.
7
[Secondary structure of the 5'-terminal region of the 18S ribosomal RNA5.3S fragment from the rat liver].[大鼠肝脏18S核糖体RNA 5.3S片段5′末端区域的二级结构]
Bioorg Khim. 1987 Jan;13(1):128-30.
8
Characteristics of nuclear-ribosomal and DNA-like ribonucleic acids differentially extracted by hot-phenol fractionation.通过热酚分级分离法差异提取的核糖体核糖核酸和类DNA核糖核酸的特性。
Eur J Biochem. 1973 May;35(1):186-200. doi: 10.1111/j.1432-1033.1973.tb02824.x.
9
The dissociation of rat liver ribosomes by formamide: extraction of a 5 S RNA-protein complex.
Methods Enzymol. 1974;30:346-9. doi: 10.1016/0076-6879(74)30036-5.
10
Incorporation of labelled precursors into the electrophoretic fractions of rat-liver ribonucleic acid.将标记前体掺入大鼠肝脏核糖核酸的电泳组分中。
Biochem J. 1966 Jul;100(1):204-10. doi: 10.1042/bj1000204.
引用本文的文献
1
Quantitative analysis of rat liver nucleolar and nucleoplasmic ribosomal ribonucleic acids.大鼠肝脏核仁与核质核糖体核糖核酸的定量分析
Biochem J. 1978 May 1;171(2):367-74. doi: 10.1042/bj1710367.
Zotero 插件