Phenol extraction of 28 S ribosomal RNA from rat liver cytoplasm.
作者信息
Dabeva M D
出版信息
Prep Biochem. 1976;06(04):285-94. doi: 10.1080/00327487608061619.
PMID:959178
Abstract
A simple and reproducible phenol method for the isolation of 28 S ribosomal RNA from rat liver cytoplasm, free from poly(A)+/-RNA is described. The procedure is based on the observation that at lower pH of the homogenate (pH 5.5) 28 S ribosomal RNA is extracted, while 18 S ribosomal RNA remains in the interphase layer.
摘要
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