Jones L B, Hardin S H
Department of Biology and Biochemistry, and Institute of Molecular Biology, University of Houston, Houston, TX 77204-5513, USA.
Nucleic Acids Res. 1998 Jun 1;26(11):2824-6. doi: 10.1093/nar/26.11.2824.
Octamer Sequencing Technology, OST, is a method of DNA sequencing using single octamer oligonucleotides to prime cycle sequencing reactions. This sequencing strategy is faster than a traditional primer-walking strategy, since access to this optimized octamer library eliminates delays associated with designing and synthesizing gene specific primers. In this report, OST has been optimized for fluorescent, dye-terminator cycle sequencing reactions to facilitate parallel processing of samples. The successful adaptation of OST to an automated sequencing platform and the design of and access to an octamer library are critical steps towards developing an efficient 'closed-loop' DNA sequencing system.
八聚体测序技术（OST）是一种利用单个八聚体寡核苷酸引发循环测序反应的DNA测序方法。这种测序策略比传统的引物步移策略更快，因为使用这个优化的八聚体文库可消除与设计和合成基因特异性引物相关的延迟。在本报告中，OST已针对荧光染料终止子循环测序反应进行了优化，以促进样品的平行处理。将OST成功应用于自动化测序平台以及八聚体文库的设计和使用是开发高效“闭环”DNA测序系统的关键步骤。
