使用质量标记的双脱氧核苷酸对单核苷酸多态性进行基质辅助激光解吸电离飞行时间质谱分型
MALDI-TOF mass spectrometric typing of single nucleotide polymorphisms with mass-tagged ddNTPs.
作者信息
Fei Z, Ono T, Smith L M
机构信息
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706-1396, USA.
出版信息
Nucleic Acids Res. 1998 Jun 1;26(11):2827-8. doi: 10.1093/nar/26.11.2827.
Abstract
A matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based method has recently been reported for the typing of single nucleotide polymorphisms using single nucleotide primer extension. This method is limited in some cases by the resolution of the mass determination, as the mass difference between nucleotides can be as little as 9 Da (the difference between A and T). A variation of this method is described here in which a mass-tagged dideoxynucleotide is employed in the primer extension reactions in place of the unmodified dideoxynucleotide. The increased mass difference due to the presence of the mass-tags substantially improves the accuracy and versatility of the procedure.
摘要
最近报道了一种基于基质辅助激光解吸/电离飞行时间质谱的方法，该方法使用单核苷酸引物延伸对单核苷酸多态性进行分型。在某些情况下，由于质量测定的分辨率，该方法受到限制，因为核苷酸之间的质量差异可能小至9道尔顿（A和T之间的差异）。本文描述了该方法的一种变体，其中在引物延伸反应中使用质量标记的双脱氧核苷酸代替未修饰的双脱氧核苷酸。由于质量标签的存在而增加的质量差异大大提高了该方法的准确性和通用性。
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