Zhu G, Marchewka M J, Ennis J G, Keithly J S
Wadsworth Center, New York State Department of Health, Albany 12208, USA.
J Infect Dis. 1998 May;177(5):1443-6. doi: 10.1086/517834.
Although polymerase chain reaction (PCR) can sensitively detect parasitic or other infections, its use with fecal samples is extremely limited, primarily because of the presence of substances that inhibit DNA extension. Here an improved protocol is reported for directly isolating DNA from aged or fresh formalin-fixed stools, which can then be used to detect Cryptosporidium parvum by nested PCR. This method is highly reproducible, sensitive, and specific. It detects <1 pg of C. parvum DNA in human stool, and there are no cross-reactions with other parasites commonly found there.
尽管聚合酶链反应（PCR）能够灵敏地检测寄生虫感染或其他感染，但其在粪便样本中的应用极为有限，主要原因是存在抑制DNA延伸的物质。本文报道了一种改进方案，可直接从陈旧或新鲜的福尔马林固定粪便中分离DNA，然后用于通过巢式PCR检测微小隐孢子虫。该方法具有高度的可重复性、敏感性和特异性。它能检测出人粪便中小于1 pg的微小隐孢子虫DNA，且与该粪便中常见的其他寄生虫无交叉反应。
