在人红细胞膜空壳中通过化学方法测定的氯-硫酸盐交换。
Chloride-sulphate exchange chemically measured in human erythrocyte ghosts.
作者信息
Romano L, Peritore D, Simone E, Sidoti A, Trischitta F, Romano P
机构信息
Ist. Fisiologia Generale, C/da Papardo, S. Agata, Messina, Italy.
出版信息
Cell Mol Biol (Noisy-le-grand). 1998 Mar;44(2):351-5.
The purpose of this paper is to provide a clear description of a new chemical method to measure and analyze the net anion exchange (Cl-/SO4--) in resealed human erythrocyte ghosts. With this technique, we measured the influx of an inorganic divalent anion (SO4--) against a monovalent anion (Cl-) as a function of temperature. We found an apparent activation enthalpy Ea of 21-24 Kcal/mol. Besides the Cl-/SO4-- influx pH - dependence, measured under condition of electric anion equilibrium, shows a bell shape with a maximum around pH 6.3. In addition we accounted for the extracellular pH changes during the exchange of chloride for sulphate across the erythrocyte ghosts when bicarbonate is present in the medium. The pH drops sharply, then increases for the remaining experimental time course.
本文的目的是清晰描述一种新的化学方法，用于测量和分析重新封闭的人红细胞血影中的净阴离子交换（Cl⁻/SO₄²⁻）。利用该技术，我们测量了无机二价阴离子（SO₄²⁻）相对于一价阴离子（Cl⁻）的流入量随温度的变化。我们发现表观活化焓Ea为21 - 24千卡/摩尔。此外，在电阴离子平衡条件下测得的Cl⁻/SO₄²⁻流入量对pH的依赖性呈钟形，在pH约6.3处有最大值。另外，当培养基中存在碳酸氢根时，我们考虑了在红细胞血影中氯离子与硫酸根交换过程中的细胞外pH变化。pH先急剧下降，然后在剩余的实验时间进程中上升。
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