Shariful Islam A T, Nakamura K, Seki T, Kuraoka A, Hirata K, Emson P C, Kawabuchi M
Department of Anatomy, Faculty of Medicine, Kyushu University, Fukuoka 812-82, Japan.
Brain Res Dev Brain Res. 1998 May 15;107(2):191-205. doi: 10.1016/s0165-3806(97)00214-9.
To investigate the possible role of nitric oxide (NO) in adult neurogenesis and neuron-glial migration in the rostral migratory stream (RMS), we used a double-labeled immunofluorescence technique together with confocal laser scanning microscopy, and examined the localization of nitric oxide synthase (NOS), the highly polysialylated isoform of neural cell adhesion molecule (PSA-N-CAM), and the astroglial marker in brain, S100 protein (S100), throughout the length of the subependymal layer (SEL) to olfactory bulb (OB) pathway of the adult guinea pig forebrain. Blast-like, beaded, clustered immature cellular elements stained for PSA-N-CAM and those having a typical astrocytic phenotypes positive for S100 protein were densely interlaced throughout the entire length of the SEL. Some S100 positive ependymoglial cells (tanycytes) gave off their basal projections into the closely packed PSA-N-CAM immunopositive clusters in the rostral extension of the subependymal zone (SEZre). The SEL was devoid of NOS immunoreactivity. A dense network of punctate, fenestrated and radially oriented immature cellular elements positive both for NOS and PSA-N-CAM intermingled and overlapped in the inner part of the internal granular layer (IGr), whereas in the outer part, PSA-N-CAM expression gradually diminished and the cells shifted to mature bipolar, spherical or spindle-shaped granule cells with uniform cellular contours, which were exclusively immunopositive for NOS. Radially oriented astroglial phenotypes were intertwined with PSA-N-CAM neuronal clusters in the SEL, and were closely apposed to NOS neuronal elements in the IGr. In summary, these results showed a distinct separation of neurons and glia as revealed by PSA-N-CAM and S100 protein immunostaining, and an inverse spatio-temporal correlation of expression between PSA-N-CAM (immature neuroblasts) and NOS (mature neurons) in the adult guinea pig RMS.
为了研究一氧化氮（NO）在成年神经发生以及吻侧迁移流（RMS）中神经元 - 神经胶质细胞迁移过程中可能发挥的作用，我们采用了双标免疫荧光技术结合共聚焦激光扫描显微镜，检测了成年豚鼠前脑从室管膜下层（SEL）到嗅球（OB）通路全程中一氧化氮合酶（NOS）、神经细胞黏附分子的高度多唾液酸化异构体（PSA - N - CAM）以及脑内星形胶质细胞标志物S100蛋白（S100）的定位情况。呈芽状、串珠状、簇状的未成熟细胞成分被PSA - N - CAM染色，而那些具有典型星形胶质细胞表型且对S100蛋白呈阳性的细胞在整个SEL全长中密集交织。一些S100阳性的室管膜胶质细胞（伸展细胞）将其基底突起伸向室管膜下区（SEZre）头端延伸部位紧密排列的PSA - N - CAM免疫阳性簇。SEL中未检测到NOS免疫反应性。在颗粒内层（IGr）内部，一个由点状、有窗孔且呈放射状排列的同时对NOS和PSA - N - CAM呈阳性的未成熟细胞成分构成的密集网络相互交织且重叠，而在其外部，PSA - N - CAM表达逐渐减少，细胞转变为具有均匀细胞轮廓的成熟双极、球形或梭形颗粒细胞，这些细胞仅对NOS呈免疫阳性。呈放射状排列的星形胶质细胞表型与SEL中的PSA - N - CAM神经元簇相互交织，并与IGr中的NOS神经元成分紧密相邻。总之，这些结果显示，通过PSA - N - CAM和S100蛋白免疫染色揭示了神经元和神经胶质细胞的明显分离，以及成年豚鼠RMS中PSA - N - CAM（未成熟神经母细胞）和NOS（成熟神经元）表达的反向时空相关性。
