Matsuyama K, Chiba Y, Sasaki M, Tanaka H, Muraoka R, Tanigawa N
Second Department of Surgery, Fukui Medical School, Japan.
Ann Thorac Surg. 1998 May;65(5):1405-9. doi: 10.1016/s0003-4975(97)01416-1.
Recent evidence suggests that tumor angiogenesis is associated with patient outcome in a number of malignancies. However, little is known about the significance of tumor microvessel density as evaluated by an anti-CD34 antibody in patients with lung cancer.
Tumor tissues were obtained from 101 patients with operable non-small cell lung cancers. Immunohistochemical detection of tumor-associated microvessels was performed using anti-CD34 antibodies directed against endothelial markers. Average counts of the three most vascular areas on a X200 field were recorded.
Staining for CD34 was easy to interpret because of specific and minimal background staining. The Kaplan-Meier method showed a statistically significant difference between the microvessel count and overall survival. The microvessel count was identified as the only significant and independent prognostic marker. In both univariate and multivariate analyses, the microvessel count was significantly related to the development of hematogenous metastasis but failed to associate with the development of nodal metastasis.
Tumor vascularity is an independent prognostic factor and important for the development of hematogenous metastasis.
最近有证据表明，肿瘤血管生成与多种恶性肿瘤患者的预后相关。然而，对于通过抗CD34抗体评估的肿瘤微血管密度在肺癌患者中的意义知之甚少。
从101例可手术的非小细胞肺癌患者中获取肿瘤组织。使用针对内皮标志物的抗CD34抗体进行肿瘤相关微血管的免疫组织化学检测。记录X200视野中三个血管最丰富区域的平均计数。
由于特异性和背景染色极少，CD34染色易于解读。Kaplan-Meier方法显示微血管计数与总生存期之间存在统计学上的显著差异。微血管计数被确定为唯一显著且独立的预后标志物。在单变量和多变量分析中，微血管计数与血行转移的发生显著相关，但与淋巴结转移的发生无关。
肿瘤血管生成是一个独立的预后因素，对血行转移的发生很重要。
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