Solé F, Woessner S, Espinet B, Lloveras E, Florensa L, Pérez-Losada A, Vilà R M, Besses C, Sans-Sabrafen J
Laboratori de Citologia Hematològica, Hospital de l'Esperança, Barcelona, Spain.
Cancer Genet Cytogenet. 1998 May;103(1):43-5. doi: 10.1016/s0165-4608(97)00358-0.
We present the cytological features, conventional cytogenetics, and in situ hybridization (ISH) findings of three cases of B-cell prolymphocytic leukemia (B-PLL). The diagnosis was made according to the French-American-British (FAB) criteria. We considered a diagnosis of B-PLL when a predominance (> 50%) of lymphoid cells with coarse chromatin but prominent central nucleoli and more abundant cytoplasm than typical chronic lymphocytic leukemia (CLL) cells were present. B-PLL express strong SIg, B-cell antigens, and reactivity with the monoclonal antibody FMC7. Chromosome analysis was carried out on lymphoid cells from peripheral blood and, in one patient, from lymph node. The phytohemagglutinin (PHA) mitogen was used. ISH was performed with two types of probes: the biotin-labeled chromosome 12-specific alpha satellite DNA probe to detect trisomy 12, and biotin-labeled libraries of whole chromosomes 1, 7, and 14. Clonal chromosome abnormalities were found in all three patients; in one, a complex karyotype was observed. The most frequent recurrent abnormality was trisomy 12. Our results suggest that PLL usually presents with cytogenetic abnormalities. The finding of translocation (11;14) is noteworthy; chromosomes 1 and 3 are also involved.
我们展示了3例B细胞幼淋巴细胞白血病（B-PLL）的细胞学特征、传统细胞遗传学及原位杂交（ISH）结果。诊断依据法国-美国-英国（FAB）标准。当存在占优势（>50%）的淋巴样细胞，其染色质粗糙但有明显的中央核仁，且细胞质比典型慢性淋巴细胞白血病（CLL）细胞更丰富时，我们考虑诊断为B-PLL。B-PLL表达强表面免疫球蛋白（SIg）、B细胞抗原，并与单克隆抗体FMC7发生反应。对来自外周血的淋巴样细胞进行了染色体分析，其中1例患者还对淋巴结细胞进行了分析。使用了植物血凝素（PHA）促有丝分裂原。ISH使用了两种类型的探针：生物素标记的12号染色体特异性α卫星DNA探针以检测12号染色体三体，以及生物素标记的1、7和14号全染色体文库。所有3例患者均发现克隆性染色体异常；其中1例观察到复杂核型。最常见的反复出现的异常是12号染色体三体。我们的结果表明，PLL通常呈现细胞遗传学异常。易位（11；14）的发现值得注意；1号和3号染色体也有涉及。
