Takahashi K, Hara E, Murakami O, Totsune K, Sone M, Satoh F, Kumabe T, Tominaga T, Kayama T, Yoshimoto T, Shibahara S
Department of Molecular Biology and Applied Physiology, Tohoku University School of Medicine, Sendai, Japan.
J Cardiovasc Pharmacol. 1998;31 Suppl 1:S367-9. doi: 10.1097/00005344-199800001-00103.
Choroid plexus carcinoma is a rare neoplasm derived from the epithelium of the choroid plexus. The production and secretion of endothelin-1 (ET-1) by cultured human choroid plexus carcinoma cells were studied by radioimmunoassay and Northern blot analysis. Immunoreactive (IR)-ET was detected in the culture medium (2.78 +/- 0.12 fmol/10(5) cells/24 h; n = 5; mean +/- SEM) but not in the unconditioned medium. Reverse-phase high-performance liquid chromatography of the extract of the culture medium showed a single peak eluting in the position of ET-1. Treatment with tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) or a combination of interferon-gamma (IFN-gamma), TNF-alpha, and IL-1 beta caused significant increases in the IR-ET levels in the culture medium. Northern blot analysis of total RNA showed the expression of ET-1 mRNA in choroid plexus carcinoma cells. The expression levels of ET-1 mRNA were increased by treatment with a combination of IFN-gamma, TNF-alpha, and IL-1 beta. The present study has shown the production and secretion of ET-1 by cultured human choroid plexus carcinoma cells and suggests the possibility that ET-1 formation is related to the pathophysiology of this tumor.
脉络丛癌是一种起源于脉络丛上皮的罕见肿瘤。采用放射免疫分析法和Northern印迹分析法研究了培养的人脉络丛癌细胞中内皮素-1（ET-1）的产生和分泌情况。在培养基中检测到免疫反应性（IR）-ET（2.78±0.12 fmol/10⁵ 细胞/24小时；n = 5；平均值±标准误），但在未处理的培养基中未检测到。对培养基提取物进行反相高效液相色谱分析，结果显示在ET-1位置有一个单一峰洗脱。用肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）或干扰素-γ（IFN-γ）、TNF-α和IL-1β的组合进行处理，可使培养基中的IR-ET水平显著升高。对总RNA进行Northern印迹分析，结果显示脉络丛癌细胞中有ET-1 mRNA的表达。用IFN-γ、TNF-α和IL-1β的组合进行处理可使ET-1 mRNA的表达水平升高。本研究表明培养的人脉络丛癌细胞可产生和分泌ET-1，并提示ET-1的形成可能与该肿瘤的病理生理学有关。
