Mack K, Titball R W
Microbiology, Chemical and Biological Defence Sector, Porton Down, Salisbury, UK.
FEMS Microbiol Lett. 1998 May 1;162(1):69-74. doi: 10.1111/j.1574-6968.1998.tb12980.x.
Using primers designed from the nucleotide sequences of five insertion elements identified previously in Burkholderia cepacia, the presence of two insertion sequences (IS406 and IS407) was detected in chromosomal DNA isolated from strains of the human pathogen Burkholderia pseudomallei. The IS407 homologue was cloned from B. pseudomallei NCTC 4845 and nucleotide sequenced to confirm its identity and degree of homology with B. cepacia IS407. A PCR amplification product from B. pseudomallei NCTC 4845 DNA provided an IS407 probe which was used to determine, by Southern blotting, the number and location of copies of IS407 in ten strains of B. pseudomallei and four representatives from three of the five genomovars of B. cepacia.
利用根据先前在洋葱伯克霍尔德菌中鉴定出的5个插入元件的核苷酸序列设计的引物，在从人类病原体类鼻疽伯克霍尔德菌菌株分离的染色体DNA中检测到了两个插入序列（IS406和IS407）。从类鼻疽伯克霍尔德菌NCTC 4845中克隆出IS407同源物，并对其进行核苷酸测序，以确认其与洋葱伯克霍尔德菌IS407的同一性和同源程度。来自类鼻疽伯克霍尔德菌NCTC 4845 DNA的PCR扩增产物提供了一个IS407探针，该探针用于通过Southern印迹法确定IS407在10株类鼻疽伯克霍尔德菌以及来自洋葱伯克霍尔德菌5个基因组变种中3个变种的4个代表菌株中的拷贝数和位置。
