空肠弯曲菌主要σ因子编码基因的克隆与特性分析。

Cloning and characterization of the gene encoding the primary sigma-factor of Campylobacter jejuni.

作者信息

Wösten M M, Boeve M, Gaastra W, van der Zeijst B A

机构信息

Department of Bacteriology, Faculty of Veterinary Medicine, Universiteit Utrecht, The Netherlands.

出版信息

FEMS Microbiol Lett. 1998 May 1;162(1):97-103. doi: 10.1111/j.1574-6968.1998.tb12984.x.

DOI:10.1111/j.1574-6968.1998.tb12984.x

PMID:9595669

Abstract

The rpoD gene encoding the primary sigma-factor of Campylobacter jejuni was amplified from genomic DNA with degenerate oligonucleotide primers. The complete gene encodes a polypeptide of 622 amino acids and has a deduced M(r) of 72.6 kDa. This polypeptide is 40% identical to the RpoD (sigma 70) protein of Escherichia coli and has 66% identity with the Helicobacter pylori RpoD protein. A C. jejuni sigma 70 promoter, not recognized by the E. coli sigma 70 factor, could be activated in this bacterium in the presence of the cloned C. jejuni RpoD protein.

摘要

使用简并寡核苷酸引物从空肠弯曲菌基因组DNA中扩增出编码主要σ因子的rpoD基因。完整基因编码一个由622个氨基酸组成的多肽，推导的分子量为72.6 kDa。该多肽与大肠杆菌的RpoD（σ70）蛋白有40%的同一性，与幽门螺杆菌的RpoD蛋白有66%的同一性。一个不被大肠杆菌σ70因子识别的空肠弯曲菌σ70启动子，在克隆的空肠弯曲菌RpoD蛋白存在的情况下可在该细菌中被激活。