Palace J, Nairne A, Hyman N, Doherty T V, Vincent A
Department of Clinical Neurology, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
Neurology. 1998 May;50(5):1463-6. doi: 10.1212/wnl.50.5.1463.
We quantified antibodies to botulinum A (anti-BTx) by immunoprecipitation of 125I-BTx. We tested seven bioassay-positive sera and 68 coded samples, including 18 from patients who had ceased to respond to BTx treatment. Compared with values from healthy control subjects and 42 neurologic control subjects, all bioassay-positive sera were positive (range, 258 to 2,809 pM) and 49 of 50 patients who continued to respond to BTx were negative (<130 pM). This simple, specific, sensitive, and quantitative assay should prove helpful in the investigation of BTx resistance.
我们通过对125I-肉毒杆菌毒素A（BTx）进行免疫沉淀来定量检测抗肉毒杆菌毒素A抗体（抗BTx）。我们检测了7份生物测定呈阳性的血清和68份编码样本，其中包括18份来自对BTx治疗不再有反应的患者的样本。与健康对照受试者和42名神经学对照受试者的值相比，所有生物测定呈阳性的血清均为阳性（范围为258至2809 pM），而50名继续对BTx有反应的患者中有49名呈阴性（<130 pM）。这种简单、特异、灵敏且定量的检测方法在研究BTx耐药性方面应会有所帮助。
