Bajaj N P, Al-Sarraj S T, Anderson V, Kibble M, Leigh N, Miller C C
Department of Clinical Neurosciences, The Institute of Psychiatry, London, UK.
Neurosci Lett. 1998 Mar 27;245(1):45-8. doi: 10.1016/s0304-3940(98)00176-1.
We have studied the distribution of cyclin dependent kinase-5 (cdk-5) within spinal cord in sporadic and two superoxide dismutase type 1 (SOD1) familial cases of amyotrophic lateral sclerosis (ALS). Although most neurofilament accumulations in ALS motor neurones did not appear to contain high levels of cdk-5, intense cdk-5 immunoreactivity was observed in perikarya of degenerating neurones in many ALS cases. Here, cdk-5 co-localised with lipofuscin. Co-localisation of cdk-5 with lipofuscin was also observed in some aged non-affected controls although this labelling was less intense than the ALS cases. The biogenesis of lipofuscin is believed to be linked to oxidative stress and oxidative stress and free radical damage have been suggested to be part of the pathogenic process of ALS, possibly involving apoptotic mechanisms. cdk-5 has recently been associated with apoptosis. These observations suggest a role for cdk-5 in the pathogenesis of ALS.
我们研究了细胞周期蛋白依赖性激酶5（cdk-5）在散发性肌萎缩侧索硬化症（ALS）以及两种1型超氧化物歧化酶（SOD1）家族性病例脊髓中的分布情况。尽管ALS运动神经元中的大多数神经丝积聚似乎并不含有高水平的cdk-5，但在许多ALS病例中，在退化神经元的胞体中观察到强烈的cdk-5免疫反应性。在此，cdk-5与脂褐素共定位。在一些未受影响的老年对照中也观察到cdk-5与脂褐素的共定位，尽管这种标记不如ALS病例强烈。脂褐素的生物合成被认为与氧化应激有关，并且氧化应激和自由基损伤被认为是ALS致病过程的一部分，可能涉及凋亡机制。cdk-5最近与凋亡相关。这些观察结果提示cdk-5在ALS发病机制中起作用。
