López-González M A, Sánchez B, Mata F, Delgado F
Unit of Pediatric Otorhinolaryngology, Virgen del Rocío University Hospital, Sevilla, Spain.
Int J Pediatr Otorhinolaryngol. 1998 Feb;43(1):33-9. doi: 10.1016/s0165-5876(97)00148-1.
Recurrent acute tonsillitis is usually produced directly by micro-organisms, mainly beta-hemolytic streptococcus. Idiopathic tonsillar hypertrophy is presented without infection history and usually leads to obstructive sleep apnea. We have measured lymphocyte subsets in tonsillar cellular suspensions of infectious and obstructive tonsillar pathology by flow cytometry. Comparing with peripheral blood, the CD4+/CD8+ ratio for tonsillar pathology varies from 4.0 to 5.0 while in peripheral blood the ratio was 1.3. In tonsils the ratio of B lymphocytes/T lymphocytes is 1.6, being 0.3 in peripheral blood, cytotoxic T lymphocytes represent 8% in tonsils and 29% in peripheral blood, virgin or nonstimulated T lymphocytes (CD4+ CD62L+) consist of 3% in tonsils and 16% in peripheral blood. The immature B lymphocytes (CD20+ CD5+) represent 23% in tonsils and 12% in peripheral blood. In regards to NK cells (CD3- CD16+), 1% was found in tonsils and 11% in peripheral blood. In tonsils B lymphocytes and a low proportion of cytotoxic T lymphocytes predominate, in comparison to peripheral blood, with a CD4+/CD8+ ratio four times greater than tonsils. We have found in tonsils a significant increase of T cells (CD3+ and TCR alpha+ beta+) in infectious processes in comparison to obstructive pathology.
复发性急性扁桃体炎通常由微生物直接引起，主要是β溶血性链球菌。特发性扁桃体肥大无感染史，通常导致阻塞性睡眠呼吸暂停。我们通过流式细胞术测量了感染性和阻塞性扁桃体病变的扁桃体细胞悬液中的淋巴细胞亚群。与外周血相比，扁桃体病变的CD4+/CD8+比值在4.0至5.0之间，而外周血中的比值为1.3。扁桃体中B淋巴细胞/T淋巴细胞的比值为1.6，外周血中为0.3，细胞毒性T淋巴细胞在扁桃体中占8%，在外周血中占29%，原始或未受刺激的T淋巴细胞（CD4+ CD62L+）在扁桃体中占3%，在外周血中占16%。未成熟B淋巴细胞（CD20+ CD5+）在扁桃体中占23%，在外周血中占12%。关于自然杀伤细胞（CD3- CD16+），在扁桃体中发现占1%，在外周血中占11%。与外周血相比，扁桃体中B淋巴细胞和低比例的细胞毒性T淋巴细胞占主导，CD4+/CD8+比值比外周血大四倍。我们发现，与阻塞性病变相比，在感染过程中扁桃体中的T细胞（CD3+和TCRα+β+）显著增加。
