Wang F, Si Y, Li Y
Reproductive Medical Centre, Tongji Medical University, Wuhan.
Zhonghua Fu Chan Ke Za Zhi. 1997 Apr;32(4):202-4.
To evaluate the effects of platelet activating factor (PAF) on the biosynthesis of thromboxane A2(TXA2) and prostaglandin I2(PGI2) in human chorionic villi and umbilical arteries.
We perfused the human placental villi and umbilical arteries with PAF (10(-9)-10(-7) mol/l), then quantified TXB2 and 6-keto-PGF1 alpha in the perfusion fluid by Radioimmunoassay.
PAF stimulated the biosynthesis of TXA2 and PGI2 in human umbilical arteries and chorionic villi in vitro. The production of TXB2 increased from 63.15 +/- 13.24 pg/mg protein to 127.03 +/- 27.76 pg/mg protein with response to the stimulation of PAF (10(-9) mol/L) (P < 0.01). The concentration of PAF (10(-8) mol/L) displayed the most optimal stimulating effect on PGI2 biosynthesis in umbilical artery samples and the production of 6-keto-PGF1 alpha increased from 6,747.52 +/- 700.31 pg/cm to 8,342 +/- 335.81 pg/cm (P < 0.01).
PAF may affect the fetal-placental circulation by regulating the local levels of PGI2 and TXA2.
评估血小板活化因子（PAF）对人绒毛膜和脐动脉中血栓素A2（TXA2）和前列腺素I2（PGI2）生物合成的影响。
用PAF（10⁻⁹ - 10⁻⁷mol/L）灌注人胎盘绒毛和脐动脉，然后通过放射免疫分析法对灌注液中的TXB2和6-酮-PGF1α进行定量。
PAF在体外刺激人脐动脉和绒毛膜中TXA2和PGI2的生物合成。随着对PAF（10⁻⁹mol/L）刺激的反应，TXB2的产生从63.15±13.24 pg/mg蛋白增加到127.03±27.76 pg/mg蛋白（P<0.01）。PAF浓度为10⁻⁸mol/L时对脐动脉样本中PGI2生物合成显示出最优化的刺激作用，6-酮-PGF1α的产生从6,747.52±700.31 pg/cm增加到8,342±335.81 pg/cm（P<0.01）。
PAF可能通过调节PGI2和TXA2的局部水平来影响胎儿-胎盘循环。
