[单纯疱疹病毒2型Sav株和野生株糖蛋白D基因的分子克隆及序列分析]
[Molecular cloning and sequence analysis of glycoprotein D gene of herpes simplex virus 2 strain Sav and wild strain isolated].
作者信息
Yang H, Li M, Jiang Y
机构信息
Guangzhou General Hospital of Guangzhou Military Command.
出版信息
Zhonghua Yi Xue Za Zhi. 1997 Feb;77(2):97-9.
OBJECTIVE
To study the difference of structure and function of HSV-2 gD gene among the wild strain, G and Sav strain. So as to provide theoretical basis for future production of HSV-2 vaccine.
METHOD
The partial glycoprotein D gene sequence of HSV-2 strain Sav and wild strain isolated from recurred genital herpes simplex were amplified and cloned with PCR.
RESULTS
The homology comparison showed that the homology of DNA and amino acid were 99.2% and 99.1% respectively.
CONCLUSIONS
There are high homology of HSV-2 gD gene among the wild strain G and Sav strains, but some variations existed.
目的
研究单纯疱疹病毒2型（HSV-2）野生株、G株和Sav株gD基因的结构与功能差异，为今后HSV-2疫苗的生产提供理论依据。
方法
采用聚合酶链反应（PCR）扩增并克隆从复发性生殖器疱疹中分离出的HSV-2 Sav株和野生株的部分糖蛋白D基因序列。
结果
同源性比较显示，DNA和氨基酸的同源性分别为99.2%和99.1%。
结论
HSV-2野生株、G株和Sav株的gD基因具有高度同源性，但存在一些变异。
Zotero 插件