Bernhard Nocht Institute for Tropical Medicine, Department of Virology, D-20359 Hamburg, Germany.
J Clin Virol. 2010 Jul;48(3):208-11. doi: 10.1016/j.jcv.2010.04.009. Epub 2010 May 21.
Recently two different herpes simplex virus type 2 (HSV-2) clades (A and B) were described on DNA sequence data of the glycoprotein E (gE), G (gG) and I (gI) genes.
To type the circulating HSV-2 wild-type strains in Germany by a novel approach and to monitor potential changes in the molecular epidemiology between 1997 and 2008.
A total of 64 clinical HSV-2 isolates were analyzed by a novel approach using the DNA sequences of the complete open reading frames of glycoprotein B (gB) and gG. Recombination analysis of the gB and gG gene sequences was performed to reveal intragenic recombinants.
Based on the phylogenetic analysis of the gB coding DNA sequence 8 of 64 (12%) isolates were classified as clade A strains and 56 of 64 (88%) isolates were classified as clade B strains. Analysis of the gG coding DNA sequence classified 4 (6%) isolates as clade A strains and 60 (94%) isolates as clade B strains. In comparison, the 8 isolates classified as clade A strains using the gB sequence data were classified as clade B strains when using the gG coding DNA sequence, suggesting intergenic recombination events. Intragenic recombination events were not detected.
The first molecular survey of clinical HSV-2 isolates from Germany demonstrated the circulation of clade A and B strains and of intergenic recombinants over a period of 12 years.
最近，根据糖蛋白 E（gE）、G（gG）和 I（gI）基因的 DNA 序列数据，描述了两种不同的单纯疱疹病毒 2 型（HSV-2）亚型（A 和 B）。
通过一种新方法对德国流行的 HSV-2 野生型毒株进行分型，并监测 1997 年至 2008 年间分子流行病学的潜在变化。
共分析了 64 例临床 HSV-2 分离株，采用新方法，利用糖蛋白 B（gB）和 gG 完整开放阅读框的 DNA 序列进行分析。对 gB 和 gG 基因序列进行重组分析，以揭示基因内重组体。
根据 gB 编码 DNA 序列的系统发生分析，64 株分离株中有 8 株（12%）被归类为 A 亚型株，56 株（88%）被归类为 B 亚型株。gG 编码 DNA 序列分析将 4 株（6%）分离株归类为 A 亚型株，60 株（94%）分离株归类为 B 亚型株。相比之下，使用 gB 序列数据分类为 A 亚型的 8 株分离株，当使用 gG 编码 DNA 序列时，被分类为 B 亚型株，这表明存在基因间重组事件。未检测到基因内重组事件。
对德国临床 HSV-2 分离株的首次分子调查表明，A 型和 B 型株以及基因间重组体在 12 年期间流行。
