Cornillot J D, Pontet M, Dupuy C, Chadli A, Caron M, Joubert-Caron R, Bourin P, Bladier D
Laboratoire de Biochimie et Technologie des Protéines (EA 1625), Université Paris Nord, UFR Santé, Médecine et Biologie Humaine, Bobigny, France.
Glycobiology. 1998 May;8(5):425-32. doi: 10.1093/glycob/8.5.425.
Antisera raised against galectin-1 exhibit crossreactivities with other galectins or related molecules. In order to overcome this problem, a monoclonal antibody to human brain galectin-1 was obtained by selecting clones without reactivity toward galectin-3. This mAb specifically bound galectin-1 of various animal origins but neither galectin-2 nor galectin-3. Western-blotting analysis of soluble human brain extracts after 2D gel electrophoresis revealed only the two most acidic isoforms of galectin-1. The ability of this mAb to bind galectin-1/asialofetuin complexes indicates that its epitope is not localized in the carbohydrate recognition domain of galectin-1. This particularity induces with efficiency its monospecificity.
针对半乳糖凝集素-1产生的抗血清与其他半乳糖凝集素或相关分子存在交叉反应。为克服这一问题，通过筛选对半乳糖凝集素-3无反应性的克隆，获得了一种针对人脑海半乳糖凝集素-1的单克隆抗体。该单克隆抗体特异性结合多种动物来源的半乳糖凝集素-1，但不结合半乳糖凝集素-2和半乳糖凝集素-3。二维凝胶电泳后对可溶性人脑海提取物进行的蛋白质免疫印迹分析仅显示了半乳糖凝集素-1的两种酸性最强的同工型。该单克隆抗体结合半乳糖凝集素-1/去唾液酸胎球蛋白复合物的能力表明其表位不在半乳糖凝集素-1的碳水化合物识别结构域中。这种特性有效地导致了其单特异性。
