Nakamura A, Watanabe T
Institute of Industrial Science, University of Tokyo, Japan.
FEBS Lett. 1998 Apr 17;426(2):201-4. doi: 10.1016/s0014-5793(98)00344-5.
The temporal evolution of pigment composition during greening of etiolated barley leaves was investigated by reversed-phase HPLC with particular attention to chlorophyll (Chl) a' (C13(2) epimer of Chl a), which had been detected by ourselves in photosystem (PS) I particles. At early stages of greening, the Chl a'/Chl a molar ratio rapidly increased to a level more than twice that in mature leaves, then gradually leveled off, accompanied by a growth of the Chl b/Chl a ratio, to the mature level. After 3 h of illumination, the temporal evolution of the Chl a'/Chl a molar ratio nearly paralleled that of the P700/Chl a ratio with a stoichiometry Chl a'/P700 approximately equal to 2: this strongly suggests that Chl a' is biosynthesized as a constituent of the PS I reaction center complex.
通过反相高效液相色谱法研究了黄化大麦叶片绿化过程中色素组成的时间演变，特别关注叶绿素（Chl）a′（Chl a的C13(2)差向异构体），我们曾在光系统（PS）I颗粒中检测到该物质。在绿化早期，Chl a′/Chl a摩尔比迅速增加至成熟叶片中该比值的两倍多，随后逐渐趋于平稳，同时Chl b/Chl a比值增长至成熟水平。光照3小时后，Chl a′/Chl a摩尔比的时间演变与P700/Chl a比值的时间演变几乎平行，化学计量比Chl a′/P700约等于2：这有力地表明Chl a′作为PS I反应中心复合物的一个组分被生物合成。
