热嗜中蓝细菌中的具法呢基尾的叶绿素 a。
Chlorophyll a with a farnesyl tail in thermophilic cyanobacteria.
机构信息
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06520-8107, USA.
Department of Chemistry, University of Connecticut, Storrs, CT, 06269-3060, USA.
出版信息
Photosynth Res. 2017 Nov;134(2):175-182. doi: 10.1007/s11120-017-0425-4. Epub 2017 Jul 24.
Photosystem II (PSII) of oxygenic photosynthetic organisms normally contains exclusively chlorophyll a (Chl a) as its major light-harvesting pigment. Chl a canonically consists of the chlorin headgroup with a 20-carbon, 4-isoprene unit, phytyl tail. We have examined the 1.9 Å crystal structure of PSII from thermophilic cyanobacteria reported by Shen and coworkers in 2012 (PDB accession of 3ARC/3WU2). A newly refined electron density map from this structure, presented here, reveals that some assignments of the cofactors may be different from those modeled in the 3ARC/3WU2 structure, including a specific Chl a that appears to have a truncated tail by one isoprene unit. We provide experimental evidence using high-performance liquid chromatography and mass spectrometry for a small population of Chl a esterified to a 15-carbon farnesyl tail in PSII of thermophilic cyanobacteria.
放氧光合作用生物的光系统 II（PSII）通常仅包含叶绿素 a（Chl a）作为其主要的光捕获色素。Chl a 通常由带有 20 个碳原子、4 个异戊二烯单元的叶绿醇尾部的氯代头部组成。我们研究了 2012 年 Shen 及其同事报道的嗜热蓝藻的 PSII 的 1.9Å 晶体结构（PDB 访问号为 3ARC/3WU2）。从这个结构中重新精修的电子密度图，在这里呈现，揭示了一些辅因子的分配可能与 3ARC/3WU2 结构中的模型不同，包括一个似乎有一个异戊二烯单元缺失的截短尾部的特定 Chl a。我们使用高效液相色谱和质谱法为嗜热蓝藻 PSII 中与 15 个碳法呢基尾部酯化的小部分 Chl a 提供了实验证据。
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