Kubo A, Minamino N, Isumi Y, Kangawa K, Dohi K, Matsuo H
National Cardiovascular Center Research Institute, Suita, Osaka, Japan.
FEBS Lett. 1998 Apr 17;426(2):233-7. doi: 10.1016/s0014-5793(98)00349-4.
We demonstrated that adrenomedullin (AM) is produced and secreted from human leukemia cell lines (THP-1 and HL-60) as well as peripheral blood granulocytes, lymphocytes, monocytes and monocyte-derived macrophages. Immunoreactive AM accumulated in the culture media of THP-1 and HL-60 cells increased according to their differentiation into macrophage-like cells. Retinoic acid exerted synergistic effects on AM secretion from THP-1 and HL-60 cells when administered with tumor necrosis factor-alpha, lipopolysaccharide or 12-O-tetradecanoyl phorbol-13-acetate. AM was shown to increase the scavenger receptor activity on THP-1 cells. Thus, monocytes/macrophages should be recognized as sources of AM, and the secreted AM may modulate the function of macrophages.
我们证明，肾上腺髓质素（AM）由人白血病细胞系（THP-1和HL-60）以及外周血粒细胞、淋巴细胞、单核细胞和单核细胞衍生的巨噬细胞产生并分泌。THP-1和HL-60细胞培养基中积累的免疫反应性AM随着它们分化为巨噬细胞样细胞而增加。当与肿瘤坏死因子-α、脂多糖或12-O-十四酰佛波醇-13-乙酸酯一起给药时，视黄酸对THP-1和HL-60细胞的AM分泌发挥协同作用。AM被证明可增加THP-1细胞上的清道夫受体活性。因此，单核细胞/巨噬细胞应被视为AM的来源，分泌的AM可能调节巨噬细胞的功能。
