Lapierre J M, Cacheux V, Da Silva F, Collot N, Hervy N, Wiss J, Tachdjian G
Service de Biochimie Génétique, Hôpital Robert Debré AP-HP, Paris, France.
Ann Genet. 1998;41(1):56-62.
Comparative genomic hybridization (CGH) offers a new global approach for detection of chromosomal material imbalances of the entire genome in a single experiment without cell culture. In this paper, we discuss the technical development and the cytogenetic aspects of CGH in a clinical laboratory. Based only on the visual inspection of CGH metaphase spreads, the correct identification of numerical and structural anomalies are reported. No commercial image analysis software was required in these experiments. We have demonstrated that this new technology can be set up easily for routine use in a clinical cytogenetics laboratory.
比较基因组杂交（CGH）提供了一种全新的整体方法，可在无需细胞培养的单个实验中检测整个基因组的染色体物质失衡情况。在本文中，我们讨论了临床实验室中CGH的技术发展及细胞遗传学方面的问题。仅基于对CGH中期染色体铺展的目视检查，就报告了对数目和结构异常的正确识别。这些实验无需商业图像分析软件。我们已经证明，这项新技术可以很容易地建立起来，用于临床细胞遗传学实验室的常规检测。
