Briant D, Ohan N, Heikkila J J
Department of Biology, University of Waterloo, ON, Canada.
Biochem Cell Biol. 1997;75(6):777-82.
We have examined the effect of herbimycin A, a benzoquinoid ansamycin antibiotic, on the pattern of gene expression in amphibians. Exposure of Xenopus laevis A6 kidney epithelial cells to 1 microgram/mL herbimycin A induced the synthesis of the heat shock proteins hsp30 and hsp70 as well as 33- and 45-kDa proteins. Enhanced synthesis of a 34-kDa protein appears to be specific to herbimycin A because its synthesis did not increase after heat shock (35 degrees C). In addition, the synthesis of hsp30 and hsp70 induced by herbimycin A was accompanied by an increase in their mRNAs. Herbimycin A induced a transient accumulation of hsp30 and hsp70 mRNA, which peaked between 4 and 6 h. Finally, concurrent treatment of cells with 0.5 microgram/mL herbimycin A and a mild heat shock of 27 degrees C yielded a synergistic accumulation of hsp30 and hsp70 mRNA. These studies demonstrate that herbimycin A can induce the expression of a set of stress proteins in amphibians and that concurrent treatment with herbimycin A and mild heat shock has a synergistic effect on the accumulation of hsp30 and hsp70 mRNA.
我们研究了苯醌安莎霉素类抗生素除草霉素A对两栖动物基因表达模式的影响。将非洲爪蟾A6肾上皮细胞暴露于1微克/毫升的除草霉素A中，可诱导热休克蛋白hsp30和hsp70以及33 kDa和45 kDa蛋白质的合成。34 kDa蛋白质合成的增强似乎是除草霉素A所特有的，因为在热休克（35℃）后其合成并未增加。此外，除草霉素A诱导的hsp30和hsp70的合成伴随着它们mRNA的增加。除草霉素A诱导hsp30和hsp70 mRNA的瞬时积累，在4至6小时达到峰值。最后，用0.5微克/毫升除草霉素A和27℃的轻度热休克同时处理细胞，可使hsp thirty和hsp70 mRNA产生协同积累。这些研究表明，除草霉素A可诱导两栖动物中一组应激蛋白的表达，并且除草霉素A与轻度热休克同时处理对hsp30和hsp70 mRNA的积累具有协同作用。
