McDowall M J, Lincoln P J, Dodd B E
Forensic Sci. 1978 Mar-Apr;11(2):155-64. doi: 10.1016/s0379-0738(78)80010-3.
The results of the detection of the Rh antigens (D, C, E, c) and the S and K antigens by an elution technique comparing the AutoAnalyser and a manual method for the detection of eluted antibody are presented. Investigation of a series of anti-D sera demonstrates that while it is not too difficult to select antisera for the detection of the D antigen in fresh stains (i.e. up to 4 weeks old) some of these sera will not be satisfactory for typing older stains. The AutoAnalyser system used is low ionic strength/polybrene which has a capacity for detecting 0.002 microgram/ml of anti-D, but in spite of this degree of sensitivity the manual technique for the detection of eluted antibody compared very favourably and tended, where older stains were being tested, to give more conclusive results than the AutoAnalyser. The superiority of the manual technique was most pronounced when detecting the S and K antigens.
本文介绍了采用洗脱技术检测Rh抗原（D、C、E、c）以及S和K抗原的结果，同时比较了自动分析仪和手动方法检测洗脱抗体的情况。对一系列抗-D血清的研究表明，虽然在新鲜血迹（即4周以内的）中选择用于检测D抗原的抗血清并不太难，但其中一些血清对于陈旧血迹的分型并不理想。所使用的自动分析仪系统是低离子强度/聚凝胺系统，其检测抗-D的能力为0.002微克/毫升，尽管有这样的灵敏度，但与手动检测洗脱抗体的技术相比，手动技术表现得非常出色，并且在检测陈旧血迹时，往往比自动分析仪给出更具决定性的结果。在检测S和K抗原时，手动技术的优越性最为明显。
