Schwartz E, Gelfand J M, Mauch J C, Kligman L H
Department of Dermatology, Mount Sinai School of Medicine, New York, New York 10029, USA.
Biochem Biophys Res Commun. 1998 May 8;246(1):217-21. doi: 10.1006/bbrc.1998.8601.
The goal of this research was to delineate the post-transcriptional mechanisms responsible for the increased elastin synthesis characteristic of sundamaged skin. In this study, a unique molecular variant of the tropoelastin mRNA transcript was identified in human sundamaged skin that was derived from the usage of an alternate polyadenylation site. Nonsolar exposed human skin expressed one tropoelastin mRNA species whereas sundamaged human skin expressed the primary tropoelastin mRNA and a larger, alternate tropoelastin mRNA formed from the utilization of a second polyadenylation site. Cultured human skin fibroblasts expressed both tropoelastin transcripts and in vitro UV treatment increased the amount of the unique tropoelastin mRNA. Hairless mouse skin (normal and UV treated) expressed the primary tropoelastin transcript although UV irradiation increased the length of its poly (A) tail two-fold. Therefore, UV radiation may stimulate elastin production by affecting polyadenylation site selection and the poly (A) tail length of tropoelastin mRNA.
本研究的目的是阐明导致日光损伤皮肤中弹性蛋白合成增加的转录后机制。在本研究中，在人类日光损伤皮肤中鉴定出原弹性蛋白mRNA转录本的一种独特分子变体，其来源于一个替代聚腺苷酸化位点的使用。未暴露于日光的人类皮肤表达一种原弹性蛋白mRNA种类，而日光损伤的人类皮肤表达主要的原弹性蛋白mRNA以及一种更大的、由第二个聚腺苷酸化位点的使用形成的替代原弹性蛋白mRNA。培养的人类皮肤成纤维细胞表达两种原弹性蛋白转录本，并且体外紫外线处理增加了独特的原弹性蛋白mRNA的量。无毛小鼠皮肤（正常和紫外线处理）表达主要的原弹性蛋白转录本，尽管紫外线照射使其聚（A）尾长度增加了两倍。因此，紫外线辐射可能通过影响聚腺苷酸化位点选择和原弹性蛋白mRNA的聚（A）尾长度来刺激弹性蛋白的产生。
