Abad-Rodríguez J, Vallejo-Cremades M, Nieto-Sampedro M
Neural Plasticity Department, Instituto Cajal, CSIC, Madrid, Spain.
Glia. 1998 Jun;23(2):156-68. doi: 10.1002/(sici)1098-1136(199806)23:2<156::aid-glia7>3.0.co;2-4.
Inhibitors of astrocyte cell division, immunologically related to the sugar moiety of epidermal growth factor receptor and to blood group antigens, have been purified from mammalian brain extracts. Mass spectra, high resolution proton magnetic resonance spectra, and chemical and enzymic analysis of the purified fraction indicated that the compounds isolated were glycosphingolipids, although signals compatible with the presence of aminoacid residues were also observed. Lectin binding indicated the presence of NAc-Neuraminic acid, NAc-glucosamine, fucose, galactose, and glucose. The inhibitor was cytostatic for astrocytes, C6 glioma cells, and endothelial cells, with approximate ID50 of 250 nM. Primary cultures of fibroblasts or 3T3 cells were not affected up to concentrations of 800 nM. Concentrations of the inhibitor of 800 nM or higher, caused non-specific cytotoxicity. The biological and immunological properties of this brain inhibitor were identical to those of the EGF receptor-related inhibitor previously described with the acronym ERI. Because of its source and cytostatic action, the glial inhibitor has been renamed neurostatin. Rabbit antibodies to neurostatin immunostained astrocytes and neurons, both in culture and in tissue sections.
已从哺乳动物脑提取物中纯化出与表皮生长因子受体糖部分及血型抗原有免疫相关性的星形胶质细胞分裂抑制剂。对纯化组分的质谱分析、高分辨率质子磁共振光谱分析以及化学和酶学分析表明，分离得到的化合物为糖鞘脂类，不过也观察到了与氨基酸残基存在相符的信号。凝集素结合表明存在N - 乙酰神经氨酸、N - 乙酰葡糖胺、岩藻糖、半乳糖和葡萄糖。该抑制剂对星形胶质细胞、C6胶质瘤细胞和内皮细胞具有细胞生长抑制作用，半数抑制浓度（ID50）约为250 nM。成纤维细胞或3T3细胞的原代培养物在浓度高达800 nM时不受影响。800 nM或更高浓度的抑制剂会引起非特异性细胞毒性。这种脑抑制剂的生物学和免疫学特性与先前所述的首字母缩写为ERI的表皮生长因子受体相关抑制剂相同。由于其来源和细胞生长抑制作用，这种胶质细胞抑制剂已被重新命名为神经抑素。抗神经抑素的兔抗体在培养物和组织切片中均对星形胶质细胞和神经元进行了免疫染色。
