Iida K, Sasa M, Serikawa T, Noda A, Ishihara K, Akimitsu T, Hanaya R, Arita K, Kurisu K
Department of Neurosurgery, Hiroshima University School of Medicine, Japan.
Epilepsy Res. 1998 Apr;30(2):115-26. doi: 10.1016/s0920-1211(97)00101-0.
Noda epileptic rat (NER) is a mutant rat, found in a Crj: Wistar colony, which exhibits a tonic clonic convulsion spontaneously about once per 30 h from 14 weeks of age. We performed modified acoustic priming, that is, repeated weekly sound stimulations from 3 weeks of age. In addition, characteristics of audiogenic seizure (AGS), and ictal/interictal electroencephalograms (EEGs) were examined. We also studied the effect of repeated weekly stimulations from 14 weeks of age on AGS susceptibility in another NER. From 9 weeks of age, the NER primed from 3 weeks of age had a high incidence (100%) of AGS: a typical seizure was composed of sudden wild running and/or jumping (WRJ) followed by clonic or tonic-clonic convulsion. The severity and the duration of the AGS were intensified and prolonged with an increase in age, respectively. By contrast, the NER repeatedly stimulated from the age of 14 weeks, rarely showed AGS (20-40(%). The majority of the seizures in this NER were WRJ. The cortical and hippocampal EEG during the tonic convulsion showed a low-voltage spike-wave (5-7 Hz). This evolved into a high-amplitude spike- or polyspike-waves associated with the clonic convulsion. Immediately after cessation of the seizures, the EEG showed a flattening or diffuse slowing. In interictal EEG analysis, sporadic spikes predominantly in the hippocampus and spike-wave bursts in both the cortex and hippocampus occurred from 11 and 20 weeks of age, respectively. These results indicate that AGS susceptibility in NER can be induced consistently by modified acoustic priming and this rat strain is a new genetic model useful for experimental studies of human epilepsy.
野田癫痫大鼠（NER）是在Crj:Wistar群体中发现的一种突变大鼠，从14周龄开始大约每30小时自发出现一次强直阵挛性惊厥。我们进行了改良的声音启动，即从3周龄开始每周重复进行声音刺激。此外，还检查了听源性惊厥（AGS）的特征以及发作期/发作间期脑电图（EEG）。我们还研究了从14周龄开始每周重复刺激对另一组NER中AGS易感性的影响。从9周龄开始，3周龄开始启动的NER发生AGS的发生率很高（100%）：典型的惊厥表现为突然疯狂奔跑和/或跳跃（WRJ），随后是阵挛性或强直阵挛性惊厥。AGS的严重程度和持续时间分别随着年龄的增长而加重和延长。相比之下，从14周龄开始反复刺激的NER很少出现AGS（20%-40%）。该组NER中的大多数惊厥为WRJ。强直惊厥期间的皮质和海马EEG显示低电压棘波（5-7Hz）。这演变成与阵挛性惊厥相关的高振幅棘波或多棘波。惊厥停止后立即，EEG显示平坦或弥漫性减慢。在发作间期EEG分析中，分别从11周龄和20周龄开始，海马中主要出现散在棘波，皮质和海马中出现棘波-慢波暴发。这些结果表明，改良的声音启动可以一致地诱导NER中的AGS易感性，并且该大鼠品系是一种可用于人类癫痫实验研究的新的遗传模型。
