Roelandse F W, Amons R, ter Braak E P, Nieuwland R, van Loon J, Souverijn J H
Department of Clinical Chemistry, Leiden University Medical Centre, Netherlands.
J Neurol Sci. 1998 Apr 15;157(1):105-8. doi: 10.1016/s0022-510x(98)00055-0.
A high alkaline fraction with a pI of 9.2 is sometimes seen on isoelectric focusing patterns of cerebrospinal fluid. The appearance of this fraction mainly depends on the type of concentrators used to prepare the cerebrospinal fluid samples, prior to isoelectric focusing. The amino acid sequence of the high alkaline fraction showed sequence identity to cystatin C, a cysteine protease inhibitor with a pI of 9.2-9.3 and a molecular mass of 13.4 kDa. In addition, on Western blot the high alkaline fraction was recognized by an antibody, directed against cystatin C. Taken together, the present findings demonstrate that the high alkaline fraction is cystatin C.
在脑脊液的等电聚焦图谱上，有时会出现一个pI为9.2的高碱性组分。该组分的出现主要取决于在等电聚焦之前用于制备脑脊液样本的浓缩器类型。高碱性组分的氨基酸序列与胱抑素C显示出序列同一性，胱抑素C是一种半胱氨酸蛋白酶抑制剂，pI为9.2 - 9.3，分子量为13.4 kDa。此外，在蛋白质印迹法中，高碱性组分可被一种针对胱抑素C的抗体识别。综上所述，目前的研究结果表明，高碱性组分就是胱抑素C。
