Engelhardt B, Martin-Simonet M T, Rott L S, Butcher E C, Michie S A
Max-Planck-Institut für physiologische und klinische Forschung, Bad Nauheim, Germany.
J Neuroimmunol. 1998 Apr 1;84(1):92-104. doi: 10.1016/s0165-5728(97)00237-3.
The phenotype of T cells in the central nervous system (CNS) in two models of chronic inflammation (experimental allergic encephalomyelitis and Corynebacterium parvum-induced inflammation) was compared to that of T cells in gut and chronically inflamed subcutaneous tissue and lung. CNS T cells display a similar phenotype in both inflammatory models, and are phenotypically unique compared to T cells from the other inflamed tissues. T cells from inflamed CNS are mainly CD4+ and are the only population examined that express a typical activated/memory phenotype: CD44high/LFA-1high/ICAM-1high/CD45RBlow. The CNS T cells are alpha4beta7-integrin(negative), but express alpha4-integrin and activated beta1 integrin, suggesting expression of the alpha4beta1-heterodimer in an activated state. In contrast, most T cells in gut express low levels of activated beta1 integrin. The CNS T cells lack expression of alpha6 and alphaE integrin chains and L-selectin. In inflamed CNS and inflamed subcutaneous tissue, approximately 50% of T cells express high affinity ligands for P-selectin while fewer than 10% express high affinity ligands for E-selectin. In summary, our data show that, independent of the inflammatory stimulus, T cells recruited into the inflamed CNS are phenotypically distinct from T cells in other inflamed tissues. This finding leads us to hypothesize the existence of a phenotypically distinct 'CNS-seeking' T lymphocyte population.
在两种慢性炎症模型（实验性变应性脑脊髓炎和微小棒状杆菌诱导的炎症）中，对中枢神经系统（CNS）中T细胞的表型与肠道、慢性炎症的皮下组织和肺中T细胞的表型进行了比较。在两种炎症模型中，CNS T细胞表现出相似的表型，并且与来自其他炎症组织的T细胞相比，其表型具有独特性。来自炎症CNS的T细胞主要为CD4 +，是所检测的唯一表达典型活化/记忆表型的群体：CD44高/LFA-1高/ICAM-1高/CD45RB低。CNS T细胞α4β7整合素呈阴性，但表达α4整合素和活化的β1整合素，提示处于活化状态的α4β1异二聚体的表达。相比之下，肠道中的大多数T细胞活化β1整合素表达水平较低。CNS T细胞缺乏α6和αE整合素链以及L-选择素的表达。在炎症CNS和炎症皮下组织中，约50%的T细胞表达P-选择素的高亲和力配体，而表达E-选择素高亲和力配体的细胞少于10%。总之，我们的数据表明，独立于炎症刺激，募集到炎症CNS中的T细胞在表型上与其他炎症组织中的T细胞不同。这一发现使我们推测存在一种表型独特的“趋向CNS”的T淋巴细胞群体。
