Edelmann L, Ruf A
Universität des Saarlandes, Homburg-Saar, Germany.
Scanning Microsc Suppl. 1996;10:295-307.
Two new simple stabilization procedures for freeze-dried biological material are introduced which are compatible with low temperature embedding (LTE) in Lowicryl. The first method uses a Lowicryl K11M/HM20 mixture supplemented with 0.3% uranyl acetate for LTE. For the second method polymerized Lowicryl blocks containing the freeze-dried material are exposed to OsO4 vapor which penetrates into the Lowicryl block and stabilizes the embedded specimen. The quality of structural preservation is demonstrated with human leukocytes.
介绍了两种用于冻干生物材料的新型简单稳定化程序，它们与在低温包埋剂（Lowicryl）中进行低温包埋（LTE）兼容。第一种方法使用添加了0.3%醋酸铀的低温包埋剂K11M/HM20混合物进行LTE。第二种方法是将含有冻干材料的聚合低温包埋剂块暴露于四氧化锇蒸汽中，蒸汽渗透到低温包埋剂块中并稳定包埋的标本。用人白细胞展示了结构保存的质量。
