Kwei S L, Clement A, Faissner A, Brandt R
Institute für Neurobiologie, Universität Heidelberg, Germany.
Neuroreport. 1998 Apr 20;9(6):1035-40. doi: 10.1097/00001756-199804200-00015.
The subcellular interactions of the neuronal microtubule-associated proteins tau, MAP2 and MAP5 were determined in cultured rat hippocampal neurons using differential detergent extraction and laser scanning microscopy. Axon development correlated with a transition from a MAP2-actin filament interaction to a MAP2-microtubule association and binding of tau to the distal axon. Tau and MAP2 binding specifically increased in the axon and the minor neurites, respectively. No compartment-specific association of MAP5 was observed. Tau binding preceded the accumulation of microtubules at the distal axon which represented a characteristic event during axogenesis. The data provide evidence for a role of MAP2 in regulating microfilament-microtubule interactions during neurite formation and of tau in organizing microtubules at the distal axon.
利用不同的去污剂提取法和激光扫描显微镜技术，在培养的大鼠海马神经元中确定了神经元微管相关蛋白tau、MAP2和MAP5的亚细胞相互作用。轴突发育与从MAP2 - 肌动蛋白丝相互作用向MAP2 - 微管结合的转变以及tau与轴突远端的结合相关。tau和MAP2的结合分别在轴突和小神经突中特异性增加。未观察到MAP5的特定区域关联。tau的结合先于微管在轴突远端的积累，这是轴突发生过程中的一个特征性事件。这些数据为MAP2在神经突形成过程中调节微丝 - 微管相互作用以及tau在轴突远端组织微管方面的作用提供了证据。
